目的:探讨p16、ESRβ蛋白在HPV相关宫颈癌中的表达及临床意义。方法:采用表面等离子谐振法检测90例宫颈癌中感染HPV类型及载量,用免疫组化方法分别检测90例宫颈癌和30例对照组宫颈组织中p16、ESRβ蛋白的表达并分析结果。结果:HPV感染类型以51亚型为主,但16亚型和18亚型的平均载量较高,与其他类型相比差异有统计学意义(P<0.05);p16蛋白的表达在宫颈癌组和对照组中的阳性率分别为100.00%和20.00%,差异有统计学意义(P<0.05);ESRβ蛋白表达的阳性率分别为14.44%和76.67%,差异有统计学意义(P<0.05)。结论:HPV、p16、ESRβ在宫颈癌的发生发展中均有重要作用,在临床工作中检测三者指标均有助于宫颈癌的诊断和预后。 Objective: To investigate the expression and clinical significance of p16 and ESRβ in HPV-related cervical cancer. Methods: Ninety cases of HPV infection in cervical cancer were detected by surface plasmon resonance. The expression of p16 and ESRβ protein in cervical tissues of 90 cases of cervical cancer and 30 cases of control group were detected by immunohistochemistry and the results were analyzed. Results: The majority of HPV infection types were 51 subtype, but the average load of 16 subtype and 18 subtype was higher than that of other types (P <0.05). The expression of p16 protein in cervical cancer (P <0.05). The positive rates of ESRβprotein expression were 14.44% and 76.67% respectively, with significant difference (P <0.05), the positive rate of ESRβprotein expression was 100.00% and 20.00% respectively ). Conclusion: HPV, p16 and ESRβ play an important role in the development of cervical cancer. The detection of three indexes in clinical work all contribute to the diagnosis and prognosis of cervical cancer.