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人巨细胞病毒(HCMV)的感染常与机体的免疫抑制有关,而此病毒对体外淋巴细胞功能是否有直接的抑制作用则尚未肯定。作者研究了新分离、传代次数不多的HCMV对NK细胞活性及T细胞增殖反应的作用,同时并与实验室的AD169株进行比较。结果表明,外周血单个核细胞与HCMV共同培养7天后,NK细胞活性受明显抑制,其抑制率可达80～100%。细胞相关病毒引起的NK细胞活性抑制比游离病毒引起的抑制强。新分离的HCMV对NK细胞活性的抑制作用比实验室毒株AD169强。NK细胞活性抑制的机理主要是由病毒本身引起,因为HCMV抗体能消除HCMV对NK细胞活性的抑制作用,使其
Human cytomegalovirus (HCMV) infection is often associated with the body’s immune suppression, and whether the virus has a direct inhibitory effect on lymphocyte function in vitro is yet to be confirmed. The authors studied the effect of HCMV, a newly isolated, less-passaged, on NK cell activity and T-cell proliferative responses, and compared it with the laboratory’s AD169 strain. The results showed that the NK cell activity was significantly inhibited after cultured with peripheral blood mononuclear cells and HCMV for 7 days, and the inhibition rate was up to 80-100%. Inhibition of NK cell activity by the cell-associated virus was stronger than that caused by the free virus. The newly isolated HCMV has a greater inhibitory effect on NK cell activity than the laboratory strain AD169. The mechanism of NK cell activity inhibition is mainly caused by the virus itself, since HCMV antibodies can eliminate the inhibitory effect of HCMV on NK cell activity, making it