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观察甘薯提取物对Aβ25-35所致SK-N-SH细胞损伤的保护作用。将人神经母细胞瘤细胞(SK-N-SH)分为空白对照组、模型组、甘薯提取物(即受试物)0.1、1.0、10.0μg/mL低、中、高剂量组和12.5μmol/L多奈哌齐阳性对照组,用20.0 mmol/L Aβ25-35造成SK-N-SH细胞损伤,MTT法测定细胞存活率,考察甘薯提取物对SK-N-SH细胞的保护作用。实验表明甘薯提取物可明显提高Aβ25-35诱导的SK-N-SH细胞存活率,并呈剂量相关性,同时能够明显改善Aβ25-35诱导的SK-N-SH损伤细胞的细胞形态变化。具有一定的神经营养及保护作用。
To observe the protective effect of sweet potato extract on Aβ25-35 induced SK-N-SH cell injury. Human neuroblastoma cells (SK-N-SH) were divided into blank control group, model group, sweet potato extract (ie, test substance) 0.1,1.0,10.0μg / mL low, middle and high dose group and 12.5μmol / L donepezil positive control group, SK-N-SH cell injury was induced by 20.0 mmol / L Aβ25-35, cell viability was determined by MTT assay, and the protective effect of sweet potato extract on SK-N-SH cells was investigated. Experiments show that sweet potato extract can significantly enhance the Aβ25-35 induced SK-N-SH cell survival rate and dose-related, and can significantly improve the Aβ25-35-induced SK-N-SH injury cell morphology changes. Has a certain neurotrophic and protective effect.