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利用硼酸与茜素红S和糖中的邻二羟基可逆结合的特点,以硼酸为中介运用竞争结合作用机理构建单糖分析法。在pH 7.4的KH2PO4-NaOH缓冲溶液中,茜素红S作为指示剂与硼酸结合生成ARS-BA配合物,其结合常数为5.09×102 L/mol。糖与指示剂ARS竞争结合硼酸使指示剂游离出来,产生明显的颜色变化,据此建立糖的识别方法。考察了D-葡萄糖、D-山梨醇、D-半乳糖、D-甘露糖、D-果糖、D-阿拉伯糖和L-阿拉伯糖对上述ARS-BA体系光谱的影响。结果显示:该体系对D-山梨醇和D-果糖有较好的光谱响应,其光谱变化灵敏度依D-山梨醇>D-果糖>D-阿拉伯糖～D-半乳糖>D-葡萄糖>D-甘露糖>L-阿拉伯糖之序。
The use of boric acid and alizarin red S and sugar adjacent ortho-dihydroxy binding characteristics of boric acid as the intermediary to use competitive binding mechanism to construct monosaccharide analysis. In pH 7.4 KH2PO4-NaOH buffer solution, alizarin red S was used as an indicator to combine with boric acid to form ARS-BA complex with a binding constant of 5.09 × 102 L / mol. Sugar and indicator ARS competitive binding boric acid indicator free, resulting in significant color changes, thus establishing a sugar recognition method. The effects of D-glucose, D-sorbitol, D-galactose, D-mannose, D-fructose, D-arabinose and L-arabinose on the spectrum of the above ARS-BA system were investigated. The results showed that the system has good spectral response to D-sorbitol and D-fructose, and the sensitivity of the system is D-sorbitol> D-fructose> D-arabinose> D-galactose> D-glucose> D- Mannose> L-arabinose order.