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原发性胆汁性肝硬化(PBC)是一种进行性、破坏性、炎症性自身免疫性肝病。血清γ球蛋白升高(以IgM为主),抗线粒体抗体阳性,尽管其发病机理还不清楚,但由免疫介导的胆管上皮细胞损伤被认为起主要作用。最近有人认为,熊去氧胆酸(UDCA)是一种治疗PBC和慢性肝炎的有效药物,具有提高胆汁酸池的亲水性、产生富含碳酸氢盐的胆汁分泌,保护肝细胞免受疏水胆汁酸损伤作用。本研究通过体外观察UDCA对产生Ig和细胞因子的影响,从而评价UDCA的免疫效应。方法:用蛋白A斑块试验(PFC)测定Ig细胞数,用ELISA测Ig量及各种细胞因子量,体外观察UDCA对Ig和淋巴因子产生的直接作用,对金萄菌
Primary biliary cirrhosis (PBC) is a progressive, destructive, inflammatory autoimmune liver disease. Serum gamma globulin is elevated (predominantly IgM) and positive for anti-mitochondrial antibodies, although immunoprecipitated bile duct epithelial cell damage is thought to play a major role, although its pathogenesis is unclear. It has recently been suggested that ursodeoxycholic acid (UDCA) is a potent drug for the treatment of PBC and chronic hepatitis with an increase in the hydrophilicity of the bile acid pool, the production of bicarbonate-rich bile secretions, the protection of hepatocytes from hydrophobic Bile acid injury. In this study, the effects of UDCA on Ig and cytokines were observed in vitro to evaluate the immune effect of UDCA. Methods: The number of Ig cells was determined by protein A plaque assay (PFC). The amount of Ig and various cytokines were measured by ELISA. The direct effects of UDCA on Ig and lymphokines were observed in vitro.