中科院微生物所陶勇课题组在大肠杆菌中表达青霉素扩环酶(DAOCS),利用全细胞催化高效转化青霉素G生成G-7-ADCA。该研究通过对大肠杆菌初级代谢网络的精巧设计,重构TCA并偶联生物合成反应,实现高效的生物转化。展示了通过重构的TCA循环来推动目标酶促反应的可能性。该研究策略在α-酮戊二酸依赖型加氧酶类以及其他与 Tao Yong, Institute of Microbiology, CAS, expressed penicillin expandase (DAOCS) in Escherichia coli and efficiently transformed G-7-ADCA with penicillin G using whole cell catalysis. This study achieved a highly efficient biotransformation through the elaborate design of the primary metabolic network of E. coli, reconstitution of TCA and coupling of biosynthetic reactions. The possibility of pushing the target enzymatic reaction through the remodeled TCA cycle is demonstrated. The research strategy in alpha-ketoglutarate-dependent oxygenases and others