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目的探讨清开灵注射液对大肠杆菌脑膜炎脑损伤的保护作用机制。方法在采用氨苄青霉素治疗兔大肠杆菌脑膜炎模型的基础上,加用清开灵注射液辅助治疗。在注菌前、注菌后16、26h分别测定脑脊液中白细胞数、蛋白质含量和脑组织水、钠、钾、钙含量,并观察脑组织中基质金属蛋白酶9(MMP9)的表达。结果清开灵注射液辅助治疗后可明显减轻单纯抗生素治疗后细菌性脑膜炎模型兔脑脊液中白细胞数、蛋白质及脑组织水、钠、钙含量的增加和钾含量的下降,减弱脑组织MMP9的表达。结论清开灵注射液辅助治疗可防止单纯抗生素治疗细菌性脑膜炎时所引起的一过性炎症反应和脑损伤加重,其机制与钙拮抗作用和减弱脑组织中MMP9的表达有关。
Objective To investigate the protective mechanism of Qingkailing injection on E. coli meningitis brain injury. Methods Based on the ampicillin treatment of E. coli meningitis model in rabbits, Qingkailing injection was used as adjuvant therapy. The number of leukocytes, protein content, and water, sodium, potassium, and calcium contents in the cerebrospinal fluid were measured before and 16 and 26 h after the injection of the bacteria. The expression of matrix metalloproteinase 9 (MMP9) in the brain tissue was observed. Results The adjuvant treatment of Qingkailing injection can significantly reduce the number of leukocytes, protein, water, sodium, and calcium in brain tissue and the decrease of potassium content in the cerebrospinal fluid of rabbits with bacterial meningitis after antibiotic treatment, and weaken the MMP9 in brain tissue. expression. Conclusion Qingkailing injection adjuvant therapy can prevent the transient inflammatory reaction and brain injury caused by antibiotics alone in the treatment of bacterial meningitis, and its mechanism is related to calcium antagonism and weakening the expression of MMP9 in brain tissue.