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为了给啤酒大麦优质高产栽培提供参考,选用甘啤4号为供试材料,采用裂区试验设计,主区为种植密度,设325万株·hm-2(D325)、375万株·hm-2(D375)和425万株·hm-2(D425)三个水平;副区为施氮量,设0(N0)、75kg·hm-2(N75)、150kg·hm-2(N150)、225kg·hm-2(N225)和300kg·hm-2(N300)五个水平,研究了密度和施氮量对啤酒大麦氮素积累、利用及籽粒蛋白质含量的影响。结果表明,种植密度仅对氮素利用效率有显著性影响,且随着密度增大氮素利用效率也增大。施氮量对籽粒清蛋白和球蛋白含量影响不显著,对植株氮素浓度、产量及籽粒醇溶蛋白含量等影响均达显著水平;随着施氮量增加,植株氮素浓度和氮素积累量均表现为先增大后减小,且均在N225处理达到最大;产量(y)随施氮量增加而增加,且与施氮量(x)可建立二次方程:y=-0.003x2+5.846 6x+2 748.7(R2=0.966 5);氮素吸收效率、氮素利用效率和氮素收获指数与施氮量均呈极显著的负相关,相关系数分别为-0.97、-0.95和-0.96;氮肥生理利用率、表观利用率和农艺利用率也在不同种植密度和施氮处理下存在差异;籽粒总蛋白、醇溶蛋白和谷蛋白含量与施氮量呈显著正相关,相关系数分别为0.99、0.97和0.99。种植密度和施氮量的互作对氮素利用效率、氮素收获指数和醇溶蛋白含量有显著影响,对其余指标的影响均不显著。
In order to provide a reference for the high-yielding and high-yielding cultivation of beer barley, Ganpi No. 4 was selected as the test material and the split plot design was adopted. The main planting density was 3.25 million hm-2 (D325) and 3.75 million hm- 2 (D375) and 4.25 million plants · hm-2 (D425) respectively. The nitrogen application rates in the subzone were 0 (N0), 75 kg · hm-2 (N75) and 150 kg · hm-2 225 kg · hm-2 (N225) and 300 kg · hm-2 (N300). The effects of nitrogen application rate and density on nitrogen accumulation, utilization and grain protein content in beer barley were studied. The results showed that the planting density had a significant effect on the nitrogen use efficiency, and the nitrogen use efficiency increased with the density increasing. Nitrogen content had no significant effect on grain albumin and globulin content, and had a significant effect on plant nitrogen concentration, grain yield and grain prolamin content. With the increase of nitrogen application rate, plant nitrogen concentration and nitrogen accumulation The results showed that all the treatments increased firstly and then decreased, and reached the maximum in N225 treatment. The yield (y) increased with the increase of nitrogen application rate, and the quadratic equation was established with the amount of nitrogen application (x): y = -0.003x2 +5.846 6x +2 748.7 (R2 = 0.966 5). The nitrogen uptake efficiency, nitrogen use efficiency and nitrogen uptake index were significantly negatively correlated with the nitrogen application rate, the correlation coefficients were -0.97, -0.95 and - 0.96; The physiological utilization, apparent utilization rate and agronomic utilization rate of nitrogen fertilizer were also different under different planting densities and nitrogen application rates. The total protein, gliadin and glutenin contents were positively correlated with nitrogen application rate, the correlation coefficient Respectively 0.99, 0.97 and 0.99. The interaction of planting density and nitrogen application had a significant effect on nitrogen use efficiency, nitrogen harvest index and prolamin content, but had no significant effect on other indexes.