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2006～2009年用DAS-ELISA方法对采自甘肃省河西地区辣(甜)椒病毒病病株的27份样本进行了烟草花叶病毒(TMV)、黄瓜花叶病毒(CMV)、烟草蚀纹病毒(TEV)、辣椒轻微斑驳病毒(PMMoV)、番茄斑萎病毒(TSWV)、马铃薯X病毒(PVX)、马铃薯Y病毒(PVY)、苜蓿花叶病毒(AMV)和蚕豆萎蔫病毒(BBWV)共9种病毒的检测,同时采用反转录聚合酶链式反应对其中20份样本进行了前4种病毒的测定。结果表明,DAS-ELISA方法检测出的病毒种类有TMV、CMV、PMMoV、PVY和TEV,其中TMV和CMV是优势毒原种群,检出率分别为44.4%和33.3%;RT-PCR方法检测出TMV、CMV、TEV和PMMoV;未检出TSWV、PVX、AMV和BBWV。
Twenty-seven samples collected from the diseased plants of spicy (sweet) pepper virus in Hexi region of Gansu Province from 2006 to 2009 were analyzed for TMV, CMV, Virus (TEV), PMMoV, TSWV, PVX, PVY, AMV and BBWV Nine kinds of viruses were detected, and the first four kinds of viruses were detected in 20 samples by reverse transcription polymerase chain reaction. The results showed that TMV, CMV, PMMoV, PVY and TEV were detected by DAS-ELISA. Among them, TMV and CMV were the dominant virulent strains with detection rates of 44.4% and 33.3%, respectively. RT-PCR detected TMV, CMV, TEV and PMMoV; TSWV, PVX, AMV and BBWV were not detected.