目的　用cDNAArray比较鼻咽癌组织及正常组织基因表达谱 ,研究鼻咽癌组织内p5 3调节基因表达差异。方法　Atlas人类肿瘤cDNA表达阵列 7742 1滤膜杂交后 ,用AtlasImage 1.0 1a分析膜杂交结果 ,RT PCR反应验证膜杂交结果 ,免疫组化证实基因在蛋白质水平的表达改变。结果　在 5 88个肿瘤相关基因中 ,共有 134个基因表达上调 ,88个基因表达下调。膜上有p5 3调节基因 32种 ,其中 13种显示差异表达 ,有 11个表达上调 ,2个表达下调。结论　在鼻咽癌组织内 ,p5 3的功能失控 ,MDM2、p2 1和Bax可能对鼻咽癌细胞的生长起重要的调控作用。 Objective To compare the gene expression profiles of nasopharyngeal carcinoma tissues and normal tissues with cDNAArray to study the difference of p5 3 regulatory gene expression in nasopharyngeal carcinoma tissues. Methods AtlasImage 1.0 1a was used to analyze the results of membrane hybridization after Atlas human tumor cDNA expression array 7742 1 hybridization. The results of membrane hybridization were verified by RT PCR and the expression of genes at protein level was confirmed by immunohistochemistry. Results Among the 588 tumor-related genes, 134 genes were up-regulated and 88 genes were down-regulated. Thirty-two p5 3 regulatory genes were found on the membrane, of which 13 showed differential expression, with 11 up-regulated and 2 down-regulated. Conclusion The function of p5 3 in nasopharyngeal carcinoma is out of control. MDM2, p21 and Bax may play an important regulatory role in the growth of NPC cells.