Objective To prepare the animal model for the interventional therapy on intracristal and membranous ventricular septal defects (VSD) without extracorporeal circulation. Method After an panplegia, 6 adult dogs were fixed in tracheal intubation, decumbent position with left chest at 45°respectively, we punctured the interventricular septum to make the preparation with the location of echocardiography (ECHO) by using the improved aorta and great saphenous vein proximal stapler-puncher. And after that, affirmed its existence also by the ECHO, measure the peak flow rate of VSD. Results Three dogs died during the operation, the VSD models were successfully set up in three survivals without extracorporeal circulation. II -III degree systolic murmur auscultated at the right sternal border. The 2 IVSD and 1 membranous VSD were examined by ECHO. The diameter of VSD ranged from 1.8 to 3.6 mm (mean 2.9 mm). The Peak Flow Rate of the VSD was ranged between 1.8 and 4.0 m/s; the pressure difference of both side of the VSD ranged between 42 and 51 mm Hg (mean 47 mm Hg, 1 mm Hg=0.133 kPa). The pulmonary arterial systolic pressure ranged between 23 to 29 mm Hg (mean 27 mm Hg). Conclusion Located by the ECHO, and without extracorporeal circulation, it is possible to prepare animal model of the intracristal and membranous VSD with the improved aorta and great saphenous vein proximal stapler-puncher. Objective To prepare the animal model for the interventional therapy on intracristal and membranous ventricular septal defects (VSD) without extracorporeal circulation. 6 adult dogs were fixed in tracheal intubation, decumbent position with left chest at 45 ° respectively, we punctured the interventricular septum to make the preparation with the location of echocardiography (ECHO) by using the improved aorta and great saphenous vein proximal stapler-puncher. And after that, affirmed its existence also by the ECHO, measure the peak flow rate of VSD. Three dogs died during the operation, the VSD models were successfully set up in three survivals without extracorporeal circulation. II-III degree systolic murmur auscultated at the right sternal border. The 2 IVSD and 1 membranous VSD were examined by ECHO. The diameter of VSD ranged from 1.8 to 3.6 mm (mean 2.9 mm) The Peak Flow Rate of the VSD was ranged between 1.8 and 4.0 m / s; the pressure difference of both sides of the VSD ranged between 42 and 51 mm Hg (mean 47 mm Hg, 1 mm Hg = 0.133 kPa). The pulmonary arterial systolic pressure ranged between 23 to 29 mm Hg ECHO, and without extracorporeal circulation, it is possible to prepare animal model of the intracristal and membranous VSD with the improved aorta and great saphenous vessel proximal stapler-puncher.