对比蛋白酶K-CTAB法、SDS-酚氯仿抽提法和试剂盒法对微污染水体总DNA的提取效果,进一步通过PCR-TGGE电泳及优势条带切胶测序分析,结果表明:SDS-酚氯仿抽提法提取水体总DNA效果最佳,试剂盒法次之,经纯化后均能扩增成功,蛋白酶K-CTAB法失败;SDS-酚氯仿抽提法比试剂盒方法的TGGE图谱条带数更多,代表的生物多样性更全面;试剂盒方法对革兰氏阳性菌的DNA提取能力不及SDS-酚氯仿抽提法。 The results of the comparison of protease K-CTAB method, SDS-phenol chloroform extraction method and kit method for total DNA extraction from micro-polluted water were further analyzed by PCR-TGGE electrophoresis and gel electrophoresis. The results showed that SDS- Extracting the total DNA of water was the best method, followed by the kit method, which could be successfully amplified after purification, and the protease K-CTAB method failed. The band number of TGGE in SDS-phenol chloroform extraction kit method More representative of the more comprehensive biological diversity; Kit method for Gram-positive bacteria DNA extraction capacity than the SDS-phenol chloroform extraction.