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目的:探讨乙酰肉豆蔻佛波酯(PMA)对Jurkat细胞可溶型TNF相关的凋亡诱导配体(solubletumornecrosisfac tor-relatedapoptosis inducingigand,sTRAIL)分泌和膜型TRAIL(membraneboundTRAIL,mTRAIL)表达的调节,以及二者的细胞毒作用。方法:分别采用ELISA及间接免疫荧光染色和流式细胞术分析,检测sTRAIL的分泌和mTRAIL的表达水平;用4h51Cr释放试验检测sTRAIL和mTRAIL对靶细胞Raji的细胞毒作用。结果:PMA刺激24h后jurkat细胞sTRAIL分泌和mTRAIL的表达均增加,sTRAIL分泌在刺激后48h达峰值,mTRAIL表达峰值在60h。两型TRAIL都具有对Raji细胞的细胞毒作用。结论:PKC活化剂PMA可上调Jurkat细胞sTRAIL分泌和mTRAIL的表达,两型TRAIL分子都具有杀伤靶细胞的细胞毒作用。
OBJECTIVE: To investigate the effect of PMA on the secretion of sTRAIL and the expression of membrane-bound TRAIL (mTRAIL) in Jurkat cells, and to investigate the role of PMA in the regulation of soluble tumor necrosis factor-related apoptosis inducing ligand (sTRAIL) The cytotoxic effect of both. Methods: The secretion of sTRAIL and the expression of mTRAIL were detected by ELISA, indirect immunofluorescence staining and flow cytometry respectively. The cytotoxic effect of sTRAIL and mTRAIL on Raji cells was detected by 4h51Cr release assay. Results: The expressions of sTRAIL and mTRAIL in Jurkat cells were increased after stimulated by PMA for 24 h. The secretion of sTRAIL reached the peak at 48 h and the peak of mTRAIL was 60 h. Both types of TRAIL have cytotoxic effects on Raji cells. CONCLUSION: PKC activator PMA can up-regulate sTRAIL secretion and mTRAIL expression in Jurkat cells. Both of the two types of TRAIL molecules have the cytotoxic effect on target cells.