用基因枪直接轰击玉米胚性愈伤组织，经潮霉素抗性选择平均每皿可得到０．７～２．０块抗性愈伤组织，且大部分可再生植株。对这些从抗性愈伤组织分化的植株进行点杂交和Ｓｏｕｔｈｅｒｎ吸印杂交的结果也证明Ｂｔ基因已插入玉米染色体组。对一个转Ｂｔ基因植株的测交后代进行了玉米螟抗性的田间鉴定，抗虫和感虫植株的比例接近１：１，符合孟德尔单显性基因的遗传规律。 Direct bombardment of maize embryogenic callus with gene gun, the selection of hygromycin resistant average of 0.7 ~ 2.0 per callus can be obtained callus, and most of the regenerable plants. Dot blotting and Southern blotting of these plants differentiated from resistant callus also demonstrated that the Bt gene has been inserted into the maize genome. The field test of resistance to Ostrinia furnacalis was carried out in the cross of Bt transgenic plants. The ratio of insect-resistant and susceptible plants was close to 1: 1, which accorded with the genetic regularity of Mendel’s dominance genes.