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对大鼠口服八珍汤后的尿液样品预处理方法进行多样性考察,以减少尿液样品中内源性杂质干扰,富集来源于八珍汤的化学成分,为八珍汤体内代谢产物的多样性分析奠定基础。分别采用水饱和的正丁醇、乙酸乙酯、SPE小柱固相萃取法对空白尿液和含药尿液进行处理,然后使用高效液相对各样品进行成分分析,通过对比经过各方法优化的尿液色谱图,确定含八珍汤的尿液样品的最佳处理方法,并据此进一步考察确定尿液的最佳收集时间段。通过分析各液相色谱图发现,在大鼠口服八珍汤后4～8 h时间段内,采用SPE小柱固相萃取法纯化的尿液样品出峰效果最好,数目最多。从而确定了含八珍汤的大鼠尿液的最优纯化方法为SPE小柱固相萃取法,最佳收集时间段为4～8 h。
After oral administration of Bazhen Tang rat urine sample pretreatment methods diversity study to reduce endogenous impurities in urine samples, enrichment of chemical composition from Bazhen Tang, Bazhen Tang metabolites The diversity analysis laid the foundation. Respectively, the water-saturated n-butanol, ethyl acetate, SPE cartridge solid-phase extraction of urine and urine samples were processed, and then use high-performance liquid samples for each component analysis, by comparing the methods optimized Urine chromatogram to determine the best treatment method of urine samples containing Bazhen Decoction, and further study to determine the best collection time period of urine. By analyzing the liquid chromatogram, urine samples purified by SPE cartridge SPE showed the best peak and the highest number in the period of 4-8 h after oral administration of Bazhen decoction in rats. Thus, the optimum purification method of rat urine containing Bazhen decoction was SPE cartridge SPE, and the optimal collection time was 4 ~ 8 h.