目的探讨通阳化浊法对心肌梗死大鼠心肌血管再生的影响。方法选取Wistar雄性大鼠80只,随机分为通阳化浊汤组、麝香保心丸组、模型组及假手术组,每组20只。通阳化浊汤组、麝香保心丸组、模型组以结扎大鼠左冠状动脉方法建立急性心肌梗死动物模型,假手术组只开胸不结扎冠状动脉。造模后24 h,通阳化浊汤组及麝香保心丸组分别灌胃给予通阳化浊汤及麝香保心丸,模型组及假手术组灌胃同等容积的生理盐水。6周末超声心动图测定左室射血分数(LVEF)、左室短轴缩短率(LVFS)、舒张末期左室内径(LVEDd),并处死大鼠。取心肌组织以免疫组织化学法染色检测缺血心肌中的微血管数(MVC)和微血管密度(MVD),因MVD以毛细血管个数/视野表示,故每例MVC值即为该大鼠的MVD值。结果 (1)模型组、麝香保心丸组及通阳化浊汤组LVEF、LVFS较假手术组降低(P均<0.05)。麝香保心丸组及通阳化浊汤组LVEF较模型组明显增高(P均<0.05),通阳化浊汤组较麝香保心丸组LVEF增高(P<0.05)。麝香保心丸组及通阳化浊汤组LVFS较模型组明显增高(P均<0.05)。模型组、麝香保心丸组及通阳化浊汤组较假手术组LVEDd增大(P均<0.05),麝香保心丸组及通阳化浊汤组LVEDd较模型组明显减小(P均<0.05)。(2)模型组、麝香保心丸组及通阳化浊汤组大鼠心肌梗死边缘区MVC和MVD较假手术组明显增多(P均<0.05),麝香保心丸组及通阳化浊汤组大鼠梗死心肌边缘区MVC和MVD较模型组明显增多(P均<0.05),通阳化浊汤组与麝香保心丸组MVC和MVD比较差异无统计学意义(P均>0.05)。结论通阳化浊法可改善心肌梗死大鼠的心功能,促进心肌梗死大鼠缺血心肌的血管再生。 Objective To investigate the effect of Tongyang Huazhuo method on myocardial revascularization in rats with myocardial infarction. Methods Eighty Wistar male rats were randomly divided into Tongyang Huazhuo Decoction group, Shexiang Baoxin Pill group, model group and sham operation group, with 20 rats in each group. Tongyang Huazhuo Decoction group and Shexiang Baoxin Pill group. The animal model of acute myocardial infarction was established by ligation of the left coronary artery in the model group, while the sham operation group only had the thoracotomy without ligation of the coronary artery. Twenty-four hours after modeling, Tongyang Huazhun Decoction group and Shexiang Baoxin Pill group were given Tongyang Huazhuo Decoction and Shexiangbaoyin Pill respectively. The model group and sham operation group were given the same volume of normal saline. Left ventricular ejection fraction (LVEF), left ventricular fractional shortening (LVFS) and left ventricular end-diastolic diameter (LVEDd) were measured by 6-week echocardiography. Rats were sacrificed. Myocardial tissue was detected by immunohistochemical staining of ischemic myocardium in the number of microvessels (MVC) and microvessel density (MVD), because of MVD in the number of capillaries / field of vision, so each MVC value is the rat’s MVD value. Results (1) LVEF and LVFS of model group, Shexiang Baoxin Pill group and Tongyang Huazhuo Decoction group were lower than those of sham operation group (all P <0.05). LVEF of Shexiang Baoxin Pill group and Tongyang Huazhoup Decoction group were significantly higher than those of model group (all P <0.05), and LVEF of Tongyang Huazhuo Decoction group was higher than that of Shexiang Baoxin Pill group (P <0.05). The LVFS in the Shexiang Baoxin Pill group and Tongyang Huazhuo Decoction group were significantly higher than those in the model group (all P <0.05). The LVEDd of model group, Shexiangbaoxin group and Tongyanghuazhuo decoction group were higher than those of sham group (all P <0.05), LVEDd of Shexiangbaoxin group and Tongyanghuazhuo decoction group were significantly lower than those of model group (P < All <0.05). (2) Compared with the sham-operation group, the MVC and MVD in the model group, the Shexiang Baoxin Pill group and the Tongyang Huazhuo Decoction group significantly increased (P <0.05), and the Shexiang Baoxin Pill group and Tongyang Huazhuo Compared with the model group, the MVC and MVD in infarcted myocardium of rats in soup group were significantly increased (all P <0.05), while there was no significant difference in MVC and MVD between Tong Yang Huazhuo Decoction group and Shexiang Baoxin Pill group (P> 0.05) . Conclusion Tongyang Huazhuo method can improve cardiac function in rats with myocardial infarction and promote angiogenesis in ischemic myocardium in rats with myocardial infarction.