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本文以猪肾组织膜偶合氧电极制成了生物组织膜细胞色素C电极,测试了电极的响应特性,在0.01～0.227mg/ml细胞色素C浓度范围内,该电极的动力学响应与细胞色素C浓度的对数间呈线性关系,相关系数为-0.9949。研究了缓冲剂、pH条件、温度、切片厚度和防腐剂等因素对电极性能的影响,并试验了组织膜的有效活性周期,采用动力学方法测定了一些样品的平均回收率为98.8%,标准偏差为3.4%。
In this paper, a biological tissue membrane cytochrome C electrode was made by porcine kidney kidney membrane coupled with oxygen electrode. The response characteristics of the electrode were tested. The kinetic response of the electrode to cytochrome C concentration ranged from 0.01 to 0.227mg / ml. The logarithm of C concentration showed a linear relationship with a correlation coefficient of -0.9949. The effects of buffer, pH, temperature, section thickness and preservative on the performance of the electrode were studied. The effective active period of the tissue membrane was tested. The average recovery of some samples was 98.8% determined by kinetic method. The standard The deviation is 3.4%.