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目的:观察柔肝抑纤饮及其加用二至丸在抗小鼠早期肝纤维化形成方面有无差异并探讨其机制。方法:建立小鼠血吸虫性肝纤维化模型,将其随机分组:A组(正常组);C组(吡喹酮组);D组(柔肝抑纤饮组);E组(柔肝抑纤饮+二至丸组)。除A组外,各组分别灌服相应药物,HE染色,VG染色,电镜进行肝组织病理学观察,检测ALT、ALB。结果:D、E组肝脏病变程度较C组明显减轻,而以E组减轻更加明显,肝窦的完整性和通透性较好;在升高ALB,降低肝组织胶原染色百分比含量等方面E组均明显优于D组(P<0.05),结论:联合二至丸后,柔肝抑纤饮抗小鼠肝纤维化的作用得以增强,提示二至丸对其具有增效、协同作用。
OBJECTIVE: To observe whether Rougan Yixian Decoction and its addition to Erzhi Pill have different effects on early liver fibrosis in mice and to explore its mechanism. METHODS: A mouse model of schistosomiasis-induced hepatic fibrosis was established and randomly divided into groups A (normal group), C (praziquantel group), D (Mugan Yixian Decoction group), and E group (roustatin) Fiber drink + two to pill group). Except for group A, each group received corresponding drugs, HE staining, VG staining, electron microscopy to observe liver pathology, and detect ALT and ALB. RESULTS: The degree of liver lesions in group D and E was significantly less than that in group C, but it was more obvious in group E. The integrity and permeability of hepatic sinusoids were better. Elevated ALB decreased the percentage of collagen staining in liver tissue. The group was significantly better than the D group (P<0.05). Conclusion: The effect of Rougan Yixian Decoction on the hepatic fibrosis in mice was enhanced in combination with Erzhi Pills, suggesting that Erzhi Pill has a synergistic and synergistic effect.