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目的:了解三七总皂甙(PNS)对新生SD大鼠窒息后脑细胞中Bax表达的影响。方法:7日健康SD大鼠88只,雌雄不拘,随机分为对照组(Ⅰ组,n=8)、窒息+NS组(Ⅱ组,n=40)和窒息+PNS组(Ⅲ组,n=40)。将新生鼠分别放入55 ml的磨口瓶中,瓶中放入5 g钠石灰,待新生鼠安静后,窒息+PNS组及窒息+NS组塞紧瓶塞30 min,取出后复氧120min,窒息后即刻分别腹腔注射PNS 100 mg.kg-1、同等容积9 g.L-1盐水,每12 h重复腹腔注射1次,注射后放回母鼠身边继续喂养,分别于窒息后6、24、48、72 h和7天处死;对照组放入55 ml磨口瓶中30 min,不塞瓶塞,取出后复氧120 min,放回母鼠身边继续喂养,6 h后全部处死。应用免疫组织化学法检测Bax蛋白的表达情况。结果:Ⅱ组和Ⅲ组各时间点的Bax蛋白表达水平比较差异有统计学意义(P<0.01)。Ⅱ组及Ⅲ组Bax蛋白表达水平明显高于对照组(P<0.01),均是48 h达高峰,然后逐渐下降,但7天时仍高于对照组,差异有统计学意义(P<0.01)。相同时间段Ⅲ组Bax蛋白表达水平明显低于Ⅱ组,差异有统计学意义(P<0.05)。结论:Bax是一种凋亡蛋白,在正常脑组织中很少表达,在窒息致新生SD大鼠缺氧缺血性脑病时表达增加,说明其在缺氧缺血性脑病中起到一定的促细胞凋亡的作用,促进脑损伤;PNS降低了凋亡蛋白Bax的表达,说明PNS可以通过减少脑组织中凋亡蛋白Bax的表达降低细胞凋亡数目,提示PNS对窒息所致的缺氧缺血性脑损伤有明显的脑保护作用。
Objective: To investigate the effects of Panax Notoginseng Saponins (PNS) on the expression of Bax in neonatal rat brain after asphyxia. Methods: Eighty - eight healthy SD rats were randomly divided into control group (group Ⅰ, n = 8), asphyxia + NS group (group Ⅱ, n = 40), asphyxia + PNS group (group Ⅲ, n = 40). The newborn rats were placed in a 55 ml mill mouth flask, the bottle into the 5 g soda lime, until the newborn rats were silent, asphyxia + PNS group and asphyxia + NS group stuffed tight stopper 30 min, remove the reoxygenation 120min , Immediately after asphyxial injection of PNS 100 mg.kg-1, the same volume of 9 gL-1 saline repeated intraperitoneal injection once every 12 h, after injection back to the mothers around to continue feeding, respectively, after asphyxia 6,24, 48, 72 h and 7 days. The control group was put in 55 ml mill mouth flask for 30 min. Without stopper, the rats were removed and reoxygenated for 120 min. Immunohistochemistry was used to detect the expression of Bax protein. Results: The expression of Bax protein in group Ⅱ and group Ⅲ was significantly different at different time points (P <0.01). The protein expression of Bax in group Ⅱ and group Ⅲ was significantly higher than that in control group (P <0.01), reached peak at 48 h and then decreased gradually, but still higher than that in control group at 7 days (P <0.01) . The expression of Bax protein in group Ⅲ was significantly lower than that in group Ⅱ at the same time point, the difference was statistically significant (P <0.05). CONCLUSION: Bax is a kind of apoptotic protein, which is rarely expressed in normal brain tissue and is expressed in asphyxiated neonatal SD rats with hypoxic-ischemic encephalopathy, indicating that Bax plays a role in hypoxic-ischemic encephalopathy PNS decreased the expression of Bax, which indicated that PNS could reduce the number of apoptotic cells by decreasing the expression of Bax in brain tissue, which indicated that PNS could inhibit apnea-induced hypoxia Ischemic brain injury has obvious brain protective effect.