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目的：建立测定车前草中高车前甙含量的高效液相色谱法。方法：以Ｎｕｃｌｅｏｓｉｌ７Ｃ１８（４．６×２５０ｍｍ，１０μ）为分析柱，流动相为甲醇水（ｐＨ３．５，１．０ｍｏｌ／Ｌ）磷酸盐缓冲液（４０∶５５∶５）；柱温４５℃；检测波长：３３５ｎｍ；流速：１．０ｍＬ／ｍｉｎ，采用面积外标法。结果：线性范围６．２５×１０－３～０．２０μｇ，平均回收率为１００．８％，ＲＳＤ为１．１９％（ｎ＝９）。结论：本法简便、灵敏、重现性好，可用于车前草中高车前甙含量测定。
Objective: To establish a high performance liquid chromatography (HPLC) method for determining the content of preharvest germanium in plantain. Methods: Nucleosil7C18 (4.6×250mm, 10μ) was used as the analytical column. The mobile phase was methanolwater(pH3.5, 1.0mol/L) phosphate buffer (40∶55∶5). Column temperature 45°C; detection wavelength: 335 nm; flow rate: 1.0 mL/min, using area external standard method. Results: The linear range was 6.25×10-3～0.20μg. The average recovery was 100.8% and the RSD was 1.19% (n=9). Conclusion: This method is simple, sensitive, and reproducible. It can be used to determine the content of pretilaine in plantain.