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为了解伯氏疟原虫(Plasmodium berghei)和约氏疟原虫(Plasmodium yoelii)感染小鼠后,对宿主的遗传、病理、生化变化的影响,我们观察了染虫后小鼠的外周血淋巴细胞微核率,精子畸形率,三种血清同工酶及肝肾的病理、生化改变。结果如下。给小白鼠(昆明株)腹腔接种鼠疟原虫。当血原虫率达60％时,取鼠尾血分离淋巴细胞,制成涂片,经Giemsa染色后观察有微核细胞数。伯氏疟原虫和约氏疟原虫感染小鼠后,淋巴细胞微核率分别为5.33‰和2.33‰,正常对照组微核率为1.2‰。伯氏疟组血微核率与对照组比较相差非常显著(P<0.01),而约氏疟组则相差不明显(P>0.05)。显示伯氏疟原虫可导致小鼠遗传损伤。
To understand the genetic, pathological and biochemical changes of the host after Plasmodium berghei infection and Plasmodium yoelii infection in mice, we observed the peripheral blood lymphocyte micronucleus in mice after infection with P. berghei and Plasmodium yoelii. Rate, sperm deformity rate, three serum isozymes, and pathological and biochemical changes in liver and kidney. The result is as follows. Mice (Kunming strain) were intraperitoneally vaccinated with Plasmodium falciparum. When the rate of blood protozoa reached 60%, lymphocytes were isolated from rat tail blood and smears were made. The number of micronucleated cells was observed after Giemsa staining. After infection with Plasmodium berghei and Plasmodium yoelii, the micronucleus frequency of lymphocytes was 5.33‰ and 2.33‰, respectively, and the micronucleus rate in normal control group was 1.2‰. The difference in the micronucleus rate between the B.subtilis malaria group and the control group was very significant (P<0.01), while that of the Y. yoelii group was not significant (P>0.05). Plasmodium berghei is shown to cause genetic damage in mice.