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为研究燕麦β-葡聚糖对高脂血症大鼠胆固醇代谢的影响,将58只雄性SD大鼠随机分为基础组(n=10,饲喂基础饲料)和模型组(n=48)。模型组饲喂高脂饲料3周后,将造模成功的高脂血症大鼠随机分为高脂对照组、低剂量组、中剂量组和高剂量组,每组10只,分别饲喂含0、6、12、24g/kgβ-葡聚糖高脂饲料。在试验进行到2、4和6周时测定大鼠的血浆总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C);在试验末期测定血浆载脂蛋白B(ApoB)和AI(ApoAI)、大鼠肝胆固醇、肝微粒体胆固醇含量、肝脏7-α羟化酶活性、粪胆固醇、粪胆汁酸和肠胆汁酸含量。结果表明,不同剂量的β-葡聚糖都极显著地降低空腹血浆TC、LDL-C和ApoB水平(p<0.01),而对HDL-C和ApoAI无显著影响。不同剂量的β-葡聚糖都显著降低肝胆固醇(p<0.05),增加微粒体胆固醇含量和7-α羟化酶活性(p<0.05),促进肠道、粪便胆汁酸和胆固醇排泄(p<0.01)。燕麦β-葡聚糖可有效降低血脂,抑制肝脏胆固醇沉积,提高7-α羟化酶活性,利于胆固醇和胆汁酸的排出,其作用效果具有剂量和时间依赖性。
To study the effect of β-glucan on cholesterol metabolism in hyperlipidemic rats, 58 male Sprague-Dawley rats were randomly divided into basal group (n = 10, basal diet) and model group (n = 48) . Three weeks after the model group was fed with high fat diet, the rats with successful model of hyperlipidemia were randomly divided into high fat control group, low dose group, medium dose group and high dose group, 10 rats in each group Containing 0,6,12,24 g / kg β-glucan high-fat feed. The levels of total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) in rats were measured at 2, 4 and 6 weeks after the test. Lipoprotein B (ApoB) and AI (ApoAI), rat hepatic cholesterol, liver microsomal cholesterol content, liver 7-alpha hydroxylase activity, fecal cholesterol, fecal bile acid and intestinal bile acid content. The results showed that different doses of β-glucan significantly reduced fasting plasma TC, LDL-C and ApoB levels (p <0.01), but had no significant effect on HDL-C and ApoAI. Different doses of β-glucan significantly reduced hepatic cholesterol (p <0.05), increased microsomal cholesterol and 7-α hydroxylase activity (p <0.05), and promoted intestinal excretion of bile acids and cholesterol <0.01). Oat β-glucan can effectively reduce blood lipids, inhibit hepatic cholesterol deposition, improve 7-α hydroxylase activity, which is conducive to the release of cholesterol and bile acids, the effect of a dose-dependent and time-dependent.