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瘤细胞在药物环境中的存活和生长能力与它的多耐药基因密切相关,已引起了病理及临床医生的广泛关注。多向耐药基因(Multi Drug Resistance MDR)本质上是一种能量依赖性“泵”,可将细胞内的药物泵出,从而降低细胞内药物浓度。是MDR在细胞膜上有一种分子量为170Ku的膜糖蛋白,称之为P-糖蛋白(P-glycoprotein,P-gp)。P-糖蛋白单克隆抗体有多种不同的克隆号,不同克隆号的单克隆抗体可以识别不同的P-gp异构体。本组选用C219和C494两种抗体,其中C219可识别细胞膜内位点,C494可识别细胞膜外位点。两者容易产生耐药的药物主要为长春花碱类和阿霉素类等。
The survival and growth ability of tumor cells in the drug environment is closely related to its multidrug resistance gene, which has aroused widespread concern of pathology and clinicians. Multi Drug Resistance MDR is essentially an energy-dependent “pump” that pumps out intracellular drugs to reduce intracellular drug concentration. MDR is a membrane glycoprotein with a molecular weight of 170Ku, which is called P-glycoprotein (P-gp). P-glycoprotein monoclonal antibodies have a variety of different cloning numbers. Different monoclonal clones can recognize different P-gp isoforms. The group selected C219 and C494 two antibodies, C219 which can identify the site within the cell membrane, C494 can identify cell membrane sites. Both drugs prone to drug resistance are mainly vinca alkaloids and doxorubicins and so on.