[Objective] The paper was to explore the antimicrobial activities of extracts from Xylaria striata via submerged culture. [Methods]The antibacterial activities of X. striata extracts against 10 kinds of bacteria were determined using Oxford cup method and microplate assay,and the antifungal activities against nine kinds of pathogenic fungi were determined using mycelial growth rate method. [Results]The inhibition zone diameters of the mycelium extract against tested bacteria ranged from 13. 0 to 17. 5 mm,and that of the fermentation broth extract ranged from 10. 5 to 12. 7 mm. Enterobacter cloacae was the most sensitive to both mycelium extract and fermentation broth extract,with the inhibition zone diameters of 17. 1 and 12. 7 mm and the minimum inhibitory concentrations of 2. 5 and 5mg / m L,respectively. The inhibitory effects of the mycelium extract and the fermentation broth extract against Fusarium graminearum were the greatest,and the inhibition rates were 89. 88% and 69. 53%,respectively. [Conclusion] X. striata has potential antimicrobial activity and deserves to be further studied. [Objective] The paper was to explore the antimicrobial activities of extracts from Xylaria striata via submerged culture. [Methods] The antibacterial activities of X. striata extracts against 10 kinds of bacteria were determined using Oxford cup method and microplate assay, and the antifungal activities against nine kinds of pathogenic fungi were determined using mycelial growth rate method. [Results] The inhibition zone diameters of the mycelium extract to tested bacteria ranged from 13. 0 to 17. 5 mm, and that of the fermentation broth extract ranged from 10. 5 to 12. 7 mm. Enterobacter cloacae was the most sensitive to both mycelium extract and fermentation broth extract, with the inhibition zone diameters of 17.1 and 12. 7 mm and the minimum inhibitory concentrations of 2.5 and 5 mg / ml , respectively. The inhibitory effects of the mycelium extract and the fermentation broth extract against Fusarium graminearum were the greatest, and the inhibition rates were 89. 88% and 69. 53%, re [Conclusion] X. striata has potential antimicrobial activity and deserves to be further studied.