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研究云南红豆杉、东北红豆杉、红豆杉、南方红豆杉和杂种红豆杉形成愈伤组织的一些因素 ,比较不同植株来源的愈伤组织系的生长特点和紫杉醇含量。通过考察愈伤组织形成的时间和比率来研究取样时间、光照条件、培养基中 2 ,4- D浓度和外植体类型对愈伤组织诱导的影响 ,用高效液相色谱法测定愈伤组织中紫杉醇含量。愈伤组织形成的迟早和比率在不同种类红豆杉之间和同一种类不同植株之间差异较大。在黑暗中 ,在添加有 0 .1mg/ L BAP和 1.0～ 3.0 m g/ L 2 ,4- D的 B5 培养基上 ,5 0 %～ 10 0 %的云南红豆杉、红豆杉、南方红豆杉、杂种红豆杉和春、夏季取样的东北红豆杉的茎段可以形成愈伤组织 ;云南红豆杉和红豆杉愈伤组织系的紫杉醇含量普遍比较高 ,因此 ,这两种红豆杉可作为获得高紫杉醇含量愈伤组织系的外植体源
Some factors were studied in the callus formation of Taxus yunnanensis, Taxus cuspidate, Taxus chinensis var. mairei, Taxus chinensis var. mairei and Taxus chinensis var. yunnanensis. The growth characteristics and paclitaxel content of callus from different plant sources were compared. The effects of sampling time, light conditions, concentration of 2,4-D in the medium and explant type on callus induction were investigated by examining the time and rate of callus formation. Callus was determined by high performance liquid chromatography. Paclitaxel content. The sooner and later the formation of callus and the ratio between the different species of yew and the same kind of different plants. In the dark, on the B5 medium supplemented with 0.1 mg/L BAP and 1.0 to 3.0 mg/L 2,4-D, 50 to 10% of Yunnan yew, Taxus chinensis, Taxus chinensis var. Callus can be formed from the hybrid yew and the stem segments of Taxus cuspidata collected from spring and summer, and the paclitaxel content of the callus lines of Taxus yunnanensis and Taxus yunnanensis is generally high. Therefore, these two yew can be used as high paclitaxel. Explant source of callus explants