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目的观察水芹(Oenanthe Javanica,OJ)水醇提取物在鸭体内对鸭乙型肝炎病毒(DHBV)-DNA 的抑制效果.方法将 DHBV 感染雏鸭随机分为高、中、低三个剂量组,分别为8,5,3g/kg 组进行药物治疗.每组5～6只,ig,2次/d×10 d;设病毒对照组(DHBV),以生理盐水(NS)代替药物,ig,2次/d×10 d.阳性药用阿昔洛韦(ACV)粉剂250 mg,ig,100mg/kg,2次/d×10 d.在感染7d 后,即用药前(TO),用药后5d(T5),用药10 d(T10)和停药后3 d(P3),自鸭腿胫静脉分别取血,分离血清,检查 DHBV-DNA 水平和肝功能.治疗结束经颈静脉放血处死动物,分别切取雏鸭肝脏,作肝病理检查.结果三批实验结果表明,水芹水醇提取物中,高剂量组对感染雏鸭无毒性,给药5,10 d 能显著降低 DHBV 感染鸭血清DHBV-DNA 水平,即 d5的测定值由1.04分别下降至0.52和0.44,抑制率分别为50.0%和50.6%;d10由0.85分别下降0.42和0.36,抑制率分别为57.7%和57.6%,与病毒对照组比较,均有显著性差异(P<0.05和 P<0.01).低剂量组对鸭血清 DHBV-DNA 也有一定疗效,并显示明显的量效关系.肝功能改善明显,给药10 d,中剂量雏鸭血中 ALT,AST,SB 含量下降率分别为44.1%,44.2%,33.3%.高剂量的下降率分别为61.0%,58.3%,64.4%.低剂量虽对肝功能有改善,但无统计显著性.肝组织病理观察证实,中、高剂量对雏鸭肝脏均有保护作用,肝细胞变性坏死均较轻,肝小叶结构基本完整.结论 OJ 在雏鸭体内有抗 DHBV 的作用,保肝效果显著.
Objective To observe the inhibitory effect of water-alcoholic extracts of Oenanthe Javanica (OJ) on duck hepatitis B virus (DHBV)-DNA. Methods DHBV-infected ducklings were randomly divided into three groups: high, medium and low doses. Drug administration was performed in groups of 8, 5, and 3 g/kg, respectively, 5 to 6 per group, ig, 2 times per day for 10 days; virus control group (DHBV) was replaced with saline (NS) instead of drugs. , 2 times / d × 10 d. Positive drug aciclovir (ACV) powder 250 mg, ig, 100 mg / kg, 2 times / d × 10 d. After 7 days of infection, before use (TO), medication After 5 days (T5), 10 days after drug administration (T10) and 3 days after drug withdrawal (P3), blood was collected from the veins of the leg veins of the duck, serum was separated, and the DHBV-DNA level and liver function were examined. Treatment was terminated by transcatheterization of the jugular vein. Animals were harvested from the livers of the ducklings for liver biopsy. The results of three batches of experiments showed that the high-dose group of the water extract of the watercress was not toxic to the infected ducklings, and the DHBV infection ducks could be significantly reduced after 5 and 10 days of administration. The serum DHBV-DNA level, that is, d5, was decreased from 1.04 to 0.52 and 0.44, and the inhibition rates were 50.0% and 50.6%, respectively; d10 decreased by 0.42 and 0.36 from 0.85, and the inhibition rates were 57.7% and 57.6%, respectively. Compared with the virus control group, there are significant Different (P<0.05 and P<0.01). The low-dose group also had a certain effect on duck serum DHBV-DNA, and showed a significant dose-effect relationship. Significant improvement in liver function, administration of 10 d, mid-dose duckling blood ALT, The reduction rates of AST and SB contents were 44.1%, 44.2%, and 33.3%, respectively. The rates of high-dose reductions were 61.0%, 58.3%, and 64.4%, respectively. Although low-dose treatment improved liver function, there was no statistical significance. Pathological observations confirmed that the medium and high doses had protective effects on the ducklings’ livers, and the degeneration and necrosis of hepatocytes were all lighter. The structure of the hepatic lobule was almost complete. Conclusion OJ has anti-DHBV effect in ducklings, and the hepatoprotective effect is significant.