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当试管内有患者血清、肝微粒体或含有肝特异抗原的可溶性部分存在时,患者周围血内淋巴细胞受有关药物刺激,可产生淋巴细胞转化或巨噬细胞移动抑制因子 (MIF)。将抗原激活的淋巴细胞制备的培养滤液注射于大鼠后,可见到胆汁流量和胆汁酸排出均有变化。制备方法和步骤:(1)从5例药源性胆郁患者取得淋巴细胞。(2)超速离心取得大鼠肝亚细胞成份和含肝特异抗原的可溶性部份,(3)采用微量法作淋巴母细胞转化试验,将鼠肝可溶性部分和可疑药物加入血细胞混
When a test tube contains patient serum, liver microsomes, or soluble fractions containing a liver-specific antigen, peripheral blood lymphocytes in the patient’s body are stimulated by the drug and produce lymphocyte transformation or macrophage migration inhibitory factor (MIF). After the culture filtrate prepared from antigen-activated lymphocytes was injected into rats, bile flow and bile acid excretion were observed. Preparation methods and steps: (1) from 5 cases of drug-induced gallbladder patients with lymphocytes. (2) ultracentrifugation to obtain rat liver sub-cellular components and liver-specific antigen-soluble part, (3) using microtome method for lymphoblast transformation test, the soluble part of the rat liver and suspicious drugs added to the blood cells mixed