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以白芥(SinapisalbaL)为母本,甘蓝(Brassica oleracea var alboglabra)为父本进行属间杂交,获得了不育及半不育的两种F1植株,再以半不育的F1植株作母本,甘蓝作父本进行回交,获得了BC1植株.利用基因组原位杂交(genomic in situ hybridization,GISH),结合双色荧光原位杂交(dual-colour fluorescence in situ hybridization,dcFISH)技术,鉴定出不育F1植株有21条染色体,其中9条来自甘蓝,12条来自白芥,属含1套甘蓝染色体及1套白芥染色体的预期杂种;半不育F1植株有30条染色体,其中18条来自甘蓝,12条来自白芥,属含2套甘蓝染色体及1套白芥染色体的非预期杂种,其花粉母细胞(PMC)减数分裂中期Ⅰ最多出现3个C-S三价体,减数分裂后期Ⅰ白芥染色体出现不同的分离比例.GISH分析结果表明,从BC1植株中鉴定出了1株甘蓝-白芥单体异附加系,其有丝分裂中期相有19条染色体,18条来自甘蓝,附加的1条来自白芥;减数分裂中期Ⅰ显示9个甘蓝的二价体及1个白芥的单价体,有时白芥的单个染色体与甘蓝的染色体形成了可能的三价体.甘蓝-白芥单体异附加系的获得为白芥基因渗入、基因定位与克隆奠定了基础.
In this study, two F1 plants, sterile and semi-sterile, were obtained by using Sinapis albaL as female parent and Brassica oleracea var alboglabra as male parent. , And the male parent of cabbage was backcrossed to obtain BC1 plants.Using genomic in situ hybridization (GISH) combined with dual-color fluorescence in situ hybridization (dcFISH) F1 plant has 21 chromosomes, of which 9 are from cabbage, 12 from white mustard, which contains 1 set of cabbage chromosomes and 1 set of white mustard chromosome expected hybrids; semi-sterile F1 plants have 30 chromosomes, of which 18 from 12 from white mustard, belong to unidentified hybrids containing 2 sets of cabbage chromosomes and 1 set of white mustard chromosomes. There were up to 3 CS triads in metaphase Ⅰ of meiosis of pollen mother cells (PMC) Ⅰ white mustard chromosome separation ratio.GISH analysis showed that from BC1 plants identified a cabbage - white mustard monomer addition system, the mitotic metaphase has 19 chromosomes, 18 from cabbage, additional 1 from white mustard; meiosis metaphase Ⅰ shows the monovalent body of nine cabbage bivalents and one of the white mustard, and sometimes the chromosomes of white mustard and cabbage form a possible trivalent body. Gene introgression, gene mapping and cloning laid the foundation.