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以‘大金星’、‘大红’、‘敞口’、‘大旺’和‘野生’山楂叶为试材,于4-10月每月采样一次,用70%乙醇提取山楂叶黄酮,研究不同发育时期山楂叶黄酮提取液的抗氧化活性。结果表明,各品种不同发育时期的叶片黄酮提取液,在相同浓度下表现出不同的抗氧化活性,各样品均具有较强的清除超氧阴离子自由基、羟自由基、DPPH自由基能力和一定的还原能力。清除超氧阴离子自由基能力远高于Vc和BHT,其中‘敞口’、‘大金星’、‘大红’均在4月清除能力最强且相近,‘大旺’和‘野生’分别在5月和6月最强,但低于前三者,栽培品种清除能力大于野生山楂;清除羟自由基能力也远高于Vc和BHT,其中‘野生’、‘大红’、‘敞口’、‘大金星’均在6月清除能力最强,‘大旺’在5月最强,野生山楂清除能力大于栽培品种;清除DPPH自由基能力都低于Vc,除‘野生’4月、5月和9月外,其余均高于或接近BHT,‘大金星’、‘敞口’、‘大旺’、‘野生’均在10月清除能力最强,‘大红’4月最强,除‘大旺’外,其他栽培品种清除能力大于野生山楂;还原能力低于Vc,与BHT相近,品种间及其不同发育时期变化不大。
Hawthorn leaves of ’Great Venus’, ’Red’, ’Exposed’, ’Dawang’ and ’Wild’ were used as test materials. Samples were taken monthly from April to October and hawthorn leaves flavones were extracted with 70% ethanol, Antioxidant Activity of Flavone Extract of Hawthorn Leaves During Development. The results showed that the flavonoids extracts of leaves at different developmental stages exhibited different antioxidant activities at the same concentration, and all the samples had strong capability of scavenging superoxide anion radical, hydroxyl radical and DPPH radical, The ability to restore. The ability of scavenging superoxide anion radical is much higher than that of Vc and BHT. Among them, ’open’, ’great Venus’ and ’red’ were the strongest and similar in April, while ’big’ and ’wild’ The strongest in the month and June, but lower than the first three, the removal ability of cultivars was greater than that of wild hawthorn; the ability of scavenging hydroxyl radical was also much higher than that of Vc and BHT, of which wild, red, open, Big Venus’ were the strongest in June, ’big wang’ was the strongest in May, and the removal capacity of wild hawthorn was higher than that of cultivars. The DPPH radical scavenging ability was lower than that of Vc, except ’wild’ in April, May and In September, the rest were higher than or close to BHT, ’Grand Venus’,’ exposure ’,’ big wang ’and’ wild ’were the strongest in October,’ red ’in April the strongest, except for’ big Wang ’, the removal ability of other cultivars was greater than that of wild hawthorn; the reducing ability was lower than that of Vc, similar to that of BHT, with little difference between varieties and their different developmental stages.