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目的 研究全反式维甲酸诱导人急性早幼粒白血病HL 6 0细胞分化机制。方法 用流式细胞仪分析维甲酸对HL 6 0细胞周期变化的影响 ,用自制的含 9984个已知基因和EST的高密度基因芯片检测HL 6 0细胞在维甲酸诱导作用下不同时期的基因表达变化。结果 HL 6 0细胞在 1μmol·L- 1 维甲酸持续作用 2 ,4和 6d时 ,流式细胞仪结果显示 4 8%～ 73%细胞阻断在G0 G1 期 ;基因表达谱分析发现 ,黏附分子、组织重建蛋白、转运蛋白、核蛋白体蛋白和涉及氧化酶激活途径的基因表达明显升高。结论 基因表达谱的研究结果表明 ,维甲酸作用HL 6 0细胞与氧化酶激活途径及组织重建蛋白的表达相关联 ,并揭示了一些已知和未知功能的基因在HL 6 0细胞分化与细胞凋亡过程中的作用。
Objective To investigate the mechanism of all-trans retinoic acid-induced human acute promyelocytic leukemia HL 60 cell differentiation. Methods The effect of retinoic acid on the cell cycle of HL 60 cells was analyzed by flow cytometry. The genes of HL 60 cells at different stages induced by retinoic acid were detected by high-density cDNA microarray with 9984 known genes and ESTs. Change of expression Results Flow cytometry showed that 48% -73% of cells were blocked in G0G1 phase in HL-60 cells treated with 1μmol·L-1-retinoic acid for 2, 4 and 6 days. Gene expression profiling showed that adhesion molecules , Tissue remodeling proteins, transporters, ribosomal proteins and genes involved in oxidative activation were significantly increased. Conclusion The results of gene expression profiling show that retinoic acid-induced HL-60 cells are associated with the activation of oxidative enzymes and the expression of tissue-engineered proteins and reveal the roles of some known and unknown genes in the differentiation and apoptosis of HL-60 cells The role of death process.