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目的:通过观察活血通督汤对脊髓缺血再灌注损伤脊髓神经细胞凋亡的影响,探讨活血通督汤改善脊髓缺血再灌注损伤的机制。方法:66只新西兰家兔随机分为假手术组、模型组、活血通督汤组。模型组、活血通督汤组采用夹闭腰动脉法制作脊髓缺血再灌注损伤模型。假手术组不夹闭腰动脉,其它步骤同模型组。采用TUNEL法检测神经细胞凋亡水平。结果:(1)苏木精-伊红染色显示:假手术组脊髓神经细胞形态基本正常;模型组和活血通督汤组各时间点可见脊髓神经细胞核固缩、深染、结构模糊,间质可见出血点;活血通督汤组各时间点脊髓神经细胞形态优于模型组。(2)TUNEL染色显示:假手术组神经细胞凋亡较少;模型组和活血通督汤组各时间点神经细胞凋亡较假手术组明显增高,再灌注24h神经细胞凋亡最多;活血通督汤组各时间点脊髓神经细胞凋亡少于模型组。结论:活血通督汤可通过减少脊髓缺血再灌注损伤中脊髓神经细胞的凋亡,从而减轻脊髓缺血再灌注损伤。
Objective: To observe the effects of Huoxuetongtu decoction on spinal cord nerve cell apoptosis after spinal cord ischemia-reperfusion injury and to explore the mechanism of Huoxuetong decoction on spinal cord ischemia-reperfusion injury. Methods: Sixty-six New Zealand rabbits were randomly divided into sham operation group, model group and Huoxue Tongsu Tang group. Model group and Huoxuetuotang group were subjected to occlusion of lumbar artery to make spinal cord ischemia-reperfusion injury model. The sham operation group did not clamp the lumbar artery, the other steps were the same as the model group. TUNEL method was used to detect neuronal apoptosis. Results: (1) Hematoxylin-eosin staining showed that the morphology of spinal cord neurons in sham operation group was basically normal. At each time point, the model group and the Huoxuetongdu decoction group showed nuclear pyknosis, deep staining, fuzzy structure, Visible bleeding; Huoxuetong decoction group at each time point spinal cord nerve cell morphology is better than the model group. (2) TUNEL staining showed that there was less apoptosis in the sham-operation group; apoptosis in the model group and Huoxuentongtang group at each time point was significantly higher than that in the sham-operated group and the apoptosis was the highest at 24 hours after reperfusion; Governor Tang group at each time point neurons apoptosis less than the model group. Conclusion: Huoxuetongdu Decoction can reduce the spinal cord ischemia-reperfusion injury by decreasing the apoptosis of spinal cord nerve cells during spinal cord ischemia-reperfusion injury.