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大肠埃希菌K1所致脑膜炎的发病率越来越高。随着抗生素耐药不断升级,治疗此致命疾病需替代方案。该研究筛选了美国国立卫生研究院(NIH)收集的小分子库,鉴定替米沙坦为一种血管紧张素Ⅱ1型受体(AT1R)阻断剂,可作为大肠埃希菌侵袭进入人脑微血管内皮细胞(HBMEC)的强效抑制剂。免疫沉淀研究表明,AT1R与内皮细胞gp96相互作用,HBMEC中的AT1R是大肠埃希菌外膜蛋白A。将HBMEC用
The incidence of meningitis caused by Escherichia coli K1 is getting higher and higher. As antibiotic resistance continues to escalate, alternative approaches to treating this deadly disease are needed. This study screened a small molecule pool collected by the National Institutes of Health (NIH) and identified telmisartan as an angiotensin II type 1 receptor (AT1R) blocker that can enter Escherichia coli into the human brain Potent inhibitor of microvascular endothelial cells (HBMECs). Immunoprecipitation studies show that AT1R interacts with endothelial cell gp96 and AT1R in HBMEC is Escherichia coli outer membrane protein A. Use HBMEC