论文部分内容阅读
作者将流感病毒H1N1株（A/PR/8/34）人流感疫苗制成免疫刺激复合物（ISCOM）或水包油（O/W）乳剂,皮下免疫BALB/c小鼠后检测其免疫原性和保护作用。ISCOM和O/W乳剂均能增强初次和二次血清抗体应答。在乳剂中加入L121非离子嵌段共聚物可进一步增强O/W乳剂疫苗的免疫应答。初次免疫后4周,以含流感病毒的气溶胶攻击小鼠,根据存活率和体重变化研究小鼠对攻击的抵抗力,结果发现抵抗力与抗体滴度有良好的相关性。
The authors tested the immunogen of influenza virus H1N1 (A / PR / 8/34) human influenza vaccine by immunostimulating complex (ISCOM) or oil-in-water (O / W) emulsion and immunizing BALB / c mice subcutaneously Sexual and protective effects. Both ISCOM and O / W emulsions enhanced both primary and secondary serum antibody responses. The addition of L121 non-ionic block copolymer to the emulsion further enhances the immune response of the O / W emulsion vaccine. Four weeks after the first immunization, the mice were challenged with the aerosol containing influenza virus, and the mice’s resistance to challenge was studied according to the survival rate and weight change. It was found that there was a good correlation between the resistance and the antibody titer.