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本实验旨在观察乳腺癌相关抗原基因1(BRCAA1)在MCF-7细胞发生发展过程中的作用,现报道如下一、材料与方1.材料:人乳腺癌细胞株MCF-7、质粒转染试剂盒、细胞增殖-毒性检测试剂盒、细胞凋亡试剂盒、BRCAA1-短发夹RNA(shRNA)质粒:BRCAA1-shRNA1(BRCAA1-homo-536):5-GCACTGATGTGAGTGCTAAATTTCAAGAGAATTTAGCACTCACA-TCAGTGCTT-3;BRCAA1-shRNA2(BRCAA1-homo-718):5-GCATATCAGGAAGCTGTTATCTTCAAGAGAGATAACAGCTTCCTGATATGCTT-3;BRCAA1-shRNA3(BRCAA1-homo-893):5-GCACTCCAGTCATAGGAAAGATTCAAGAGATCTTTCCTATGACTGGAGTGCTT-3;BRCAA1-shRNA4 (BRCAA1-homo-1371):5-GGAGGATAATAGCAGTGAAGATTCAAGAGATCTTCACTGCTATTATCCTCCTT-3.