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目的:为获得质量分数较高的蛇葡萄素原料,建立大孔吸附树脂分离纯化显齿蛇葡萄叶中蛇葡萄素的方法。方法:比较6种不同型号大孔吸附树脂的静态吸附与解吸附能力,考察动态吸附与洗脱参数,筛选最佳大孔吸附树脂分离纯化蛇葡萄素的工艺条件。结果:优选SP700型大孔吸附树脂为最佳型号树脂,并确定径高比为1∶10,上样浓度为5 mg/m L,上样体积为4 BV,上样速度为1 BV/h。以2 BV/h的流速分别用水(7 BV)、10%乙醇(8 BV)、20%乙醇(11 BV)进行动态洗脱,最终获得质量分数达95.5%的蛇葡萄素。结论:大孔吸附树脂法操作简单、可行,可获得高质量分数的蛇葡萄素原料。
OBJECTIVE: To establish a macroporous adsorption resin for the separation and purification of amygdalin from grape leaves of Hippophae leucocephala in order to obtain the raw material of amygdaloid with higher mass fraction. Methods: The static adsorption and desorption capacities of six kinds of macroporous adsorption resins were compared. The dynamic adsorption and elution parameters were investigated, and the optimum conditions for the separation and purification of the ophthalmophyll from the macroporous adsorption resin were screened. Results: The optimum model resin was selected as SP700 macroporous resin. The diameter-height ratio was 1:10, the loading concentration was 5 mg / mL, the loading volume was 4 BV, and the loading speed was 1 BV / h . The elution was carried out at a flow rate of 2 BV / h with water (7 BV), 10% ethanol (8 BV) and 20% ethanol (11 BV), respectively. Conclusion: The macroporous adsorption resin method is simple and feasible to obtain the high quality fraction of snake grape raw material.