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目的研究海洋来源真菌Penicillium sacculum的次级代谢产物。方法采用多种色谱方法进行分离纯化,通过理化性质和波谱数据鉴定化合物的结构;采用台盼蓝排斥法和MTT法进行抗肿瘤活性测试。结果从发酵液和菌丝体中共分离得到10个化合物,结构鉴定为(R)-1,3-二氢-6-甲氧基-3-甲基异苯并呋喃-4,5-二醇(1)、灰黄霉素(2)、1,6-二羟基-3-甲氧基-8-甲基酮(3)、1-羟基-3-甲基酮(4)、1,3,6-三羟基-8-甲基酮(5)、1,8-二羟基-3-甲基蒽醌(6)、1,3,5-三羟基-7-甲基蒽醌(7)、6-羟基芦荟大黄素(8)、腺嘌呤(9)、胸腺嘧啶(10);化合物1和3对人急性髓细胞性白血病细胞HL-60分别具有较强和中等强度的抑制活性。结论化合物1和4均为首次从该属真菌中分离得到;首次对化合物1的1H-NMR和13C-NMR谱信号进行了全归属;化合物1和3具有抗肿瘤活性。
Objective To study the secondary metabolites of marine fungi Penicillium sacculum. Methods The compounds were separated and purified by a variety of chromatographic methods. The structures of compounds were identified by physico-chemical properties and spectral data. The antitumor activity was determined by trypan blue exclusion and MTT assay. Results Ten compounds were isolated from the fermentation broth and mycelia. Their structures were identified as (R) -1,3-dihydro-6-methoxy-3-methylisobenzofuran-4,5-diol (1) griseofulvin (2) 1,6-dihydroxy-3-methoxy-8-methyl ketone (3) , 6-trihydroxy-8-methyl ketone (5), 1,8-dihydroxy-3-methylanthraquinone (6), 1,3,5-trihydroxy- , 6-hydroxy aloe-emodin (8), adenine (9) and thymine (10). Compounds 1 and 3 showed strong and moderate inhibitory activity on human acute myelogenous leukemia cells HL-60, respectively. CONCLUSION Compounds 1 and 4 were isolated from this fungus for the first time. 1H-NMR and 13C-NMR spectra of compound 1 were completely assigned for the first time. Compounds 1 and 3 showed antitumor activity.