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目的探讨水的氯化消毒副产物二溴乙酸(DBA)对BALB/c小鼠免疫功能的影响。方法 BALB/c小鼠按体重随机分为5组,每组10只,雌雄各半。分别为去离子水(阴性对照组)、环磷酰胺10 mg/kg(阳性对照组)和DBA5、20和50 mg/kg,采用等体积灌胃法(0.1 ml/10g.BW),连续灌胃28 d。观察小鼠体重变化,胸腺和脾脏的病理学改变,检测巨噬细胞的吞噬功能以及NK细胞杀伤活性、T和B淋巴细胞体外增殖功能以及血清IgG的含量。结果与阴性对照组比较,DBA灌胃组小鼠体重差异无统计学意义;脾脏脏器系数增加,胸腺脏器系数降低,差异均具有统计学意义(P<0.05);脾和胸腺均发生明显的病理学改变;随着DBA染毒剂量的增加,小鼠巨噬细胞吞噬能力、NK细胞的杀伤活性、T、B淋巴细胞的体外增殖能力和血清中IgG抗体浓度逐渐降低,并存在剂量-反应关系,差异具有统计学意义(P<0.05)。结论 DBA对小鼠的免疫功能具有明显的抑制作用。
Objective To investigate the effect of chlorination disinfection byproduct dibromoacetic acid (DBA) on immune function in BALB / c mice. Methods BALB / c mice were randomly divided into 5 groups according to body weight, with 10 mice in each group. They were treated with deionized water (negative control group), cyclophosphamide 10 mg / kg (positive control group) and DBA 5, 20 and 50 mg / kg respectively. Stomach 28 d. The changes of body weight, the pathological changes of thymus and spleen were observed. The phagocytosis of macrophages and NK cell killing activity, the proliferation of T and B lymphocytes in vitro and the content of serum IgG were also observed. Results Compared with the negative control group, there was no significant difference in body weight between the DBA group and the control group (P <0.05). The spleen organ coefficient increased and the thymus organ coefficient decreased (P <0.05) With the increase of DBA dose, phagocytosis of mouse macrophages, cytotoxicity of NK cells, proliferation ability of T and B lymphocytes in vitro and serum IgG antibody concentration gradually decreased, and there was dose- Response relationship, the difference was statistically significant (P <0.05). Conclusion DBA has a significant inhibitory effect on immune function in mice.