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活性氧(active oxygen species,AOS)在植物抗病中发挥着重要作用,主要由NADPH氧化酶(nicotinamide adenine dinucleotide phosphate oxidase)系统产生。为明确NADPH氧化酶NbRboh B基因在本氏烟与疫霉菌亲和与非亲和性互作中的功能,采用荧光定量PCR技术以及病毒诱导的基因沉默方法探究了NbRboh B基因在本氏烟中对2种疫霉菌抗性中的作用,并利用NADPH氧化酶抑制剂对辣椒疫霉的抗性进行了检测。结果发现:2种疫霉菌均能诱导本氏烟发生氧迸发,且NbRboh B基因可能参与了疫霉菌诱导本氏烟发生的氧迸发过程。该基因沉默后降低了本氏烟对亲和互作辣椒疫霉菌的抗性,但对非亲和互作疫霉菌的抗性没有肉眼可见的影响;NADPH氧化酶抑制剂处理本氏烟后也能降低其对辣椒疫霉的抗性。表明该基因通过介导AOS产生,参与植物对亲和性与非亲和性互作疫霉的抗病反应,在亲和互作中尤为重要。
Active oxygen species (AOS) plays an important role in plant disease resistance and is mainly produced by the system of nicotinamide adenine dinucleotide phosphate oxidase. In order to clarify the function of NADPH oxidase NbRboh B gene in affinity and nonaffinity interaction of N. benthamiana with Phytophthora capsici, fluorescent quantitative PCR and virus-induced gene silencing were used to explore the role of NbRboh B gene in N. benthamiana On the resistance of two kinds of Phytophthora capsici, and tested the resistance to Phytophthora capsici with NADPH oxidase inhibitor. The results showed that both Phytophthora infants could induce Oxygen burst in N. benthamiana, and NbRboh B gene may be involved in the oxygen burst of N. benthamiana induced by Phytophthora capsici. The gene silencing reduced the resistance of N. benthamiana to affinity-interacting Phytophthora capsici, but no visible effect on non-affinity interaction of Phytophthora infestans; NADPH oxidase inhibitor treatment of N. benthamiana Can reduce its resistance to Phytophthora capsici. The results indicated that this gene is particularly important in affinity interaction by mediating the production of AOS and participating in the plant resistance to both affinity and non-affinity P. philoxeroides.