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目的 观察单一TTV(transfusiontrsnsmittedvirus)急性感染患者肝组织病理损害特点及病毒DNA的表达。方法 对某职业学校TTV感染流行期间 ,18例血清TTV阳性住院患者的肝活检组织进行HE、浸银Gomori染色检查 ,以及地高辛标记TTVDNA(Dig TTVDNA)探针原位杂交。结果 18例肝穿刺标本中出现汇管区炎 14例(77 8% ) ,胆小管损害 6例 (33 4% ) ,肝小叶内灶状坏死 7例 (38.9% ) ,微脂滴 3例 (16 7% ) ,汇管区纤维化 2例(11 1% )。原位杂交阳性 8例 (4 4 4% )。TTV阳性表达细胞散在分布于肝小叶内 ,坏死区及汇管区旁较密集。结论 单一TTV急性感染可致患者肝组织轻度炎性病变 ,其特征是汇管区炎及胆小管损害。TTV可能是一种嗜肝病毒
Objective To observe the pathological features of liver tissue and the expression of viral DNA in patients with acute infection of single TTV (transfusiontrsnsmittedvirus). Methods During the prevalence of TTV infection in a vocational school, liver biopsies of 18 TTV positive inpatients were examined by HE staining, Gomori staining with immersion silver and in situ hybridization with DigTVDNA probe. Results Eighteen patients with hepatic puncture showed 14 cases (77.8%) of colitis, 6 cases of tubule damage (33.4%), 7 cases of hepatic lobule focal necrosis (38.9%), 3 cases of micro-lipid droplets 16 7%), portal fibrosis in 2 cases (11 1%). In situ hybridization in 8 cases (444%). TTV positive cells scattered scattered in the hepatic lobule, necrotic area and portal area next to the more dense. Conclusions Acute TTV infection can cause mild inflammatory lesions in liver tissue, which are characterized by inflammation of the portal area and damage of the bile ducts. TTV may be a hepadnavirus