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目的:观察蛋白酶体抑制剂MG132联合顺铂对人宫颈癌Hela细胞裸鼠移植瘤的影响。方法:建立人宫颈癌裸鼠移植瘤模型,将荷瘤鼠分成4组,每组5只,分别给予腹腔注射。对照组:生理盐水0.2 ml,1次.d-1,共7 d。MG132组:5 mg.kg-1MG132,0.2 ml,1次.d-1,共7 d。顺铂组:3 mg.kg-1顺铂,0.2 ml,第3、5、7天,1次.d-1。MG132联合顺铂组:5 mg.kg-1MG132,0.2 ml,1次.d-1,共7 d;3 mg.kg-1顺铂,0.2 ml,第3、5、7天,1次.d-1。计算瘤体积,绘制瘤体生长曲线;称瘤重,计算抑瘤率及瘤体抑制率,比较各组荷瘤鼠瘤体积和瘤重的差异。结果:各组荷瘤鼠瘤体积两两比较差异均有统计学意义(P<0.001),MG132组、顺铂组以及MG132联合顺铂组的瘤体生长较对照组明显缓慢。荷瘤鼠瘤重各组间两两比较差异均有统计学意义(P<0.001),各干预组的荷瘤鼠瘤重比对照组明显减轻。MG132组、顺铂组、MG132联合顺铂组荷瘤鼠抑瘤率分别为15%、48%和81%;MG132组、顺铂组、MG132联合顺铂组荷瘤鼠瘤重抑制率分别为9%、43%和69%。结论:MG132能抑制裸鼠体内Hela细胞移植瘤的生长,并增强顺铂对裸鼠Hela细胞移植瘤生长的抑制作用。
Objective: To observe the effect of proteasome inhibitor MG132 combined with cisplatin on transplanted human cervical carcinoma Hela cells in nude mice. Methods: To establish human cervical carcinoma xenograft model in nude mice. The tumor-bearing mice were divided into 4 groups with 5 mice in each group, which were given intraperitoneal injection respectively. Control group: saline 0.2 ml, once .d-1, a total of 7 d. MG132 group: 5 mg.kg-1MG132, 0.2 ml, once .d-1 for 7 days. Cisplatin group: 3 mg.kg-1 cisplatin, 0.2 ml, on the 3rd, 5th, 7th day, once .d-1. MG132 combined with cisplatin group: 5 mg.kg-1MG132, 0.2 ml, once .d-1, a total of 7 days; 3 mg.kg-1 cisplatin, 0.2 ml, the first 3,5,7 days, d-1. Calculate the tumor volume, draw the growth curve of the tumor; Weigh the tumor weight, calculate the tumor inhibition rate and the inhibition rate of the tumor, compare the difference of tumor volume and tumor weight between the groups. Results: There was significant difference in tumor size between groups (P <0.001). The tumor growth of MG132 group, cisplatin group and MG132 combined with cisplatin group was significantly slower than that of control group. The tumor weight of tumor-bearing mice was significantly different between groups (P <0.001), and the tumor weight of tumor-bearing mice in each intervention group was significantly lower than that of the control group. MG132 group, cisplatin group, MG132 combined with cisplatin group tumor-bearing mice inhibition rates were 15%, 48% and 81%; MG132 group, cisplatin group, MG132 combined cisplatin group tumor bearing tumor weight inhibition rates were 9%, 43% and 69%. CONCLUSION: MG132 can inhibit the growth of Hela cell xenografts in nude mice and enhance the inhibitory effect of cisplatin on the growth of Hela cell xenografts in nude mice.