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人子宫颈鳞状上皮癌(简称宫颈癌)是妇女死亡的主要原因之一,近年来,实验室的研究证明了某些类型的人乳头瘤病毒(Human Papillomavirus,HPV)与宫颈癌以及其它几种生殖肿瘤病有明显的关系。本文应用多重引物与共有引物PCR法对山西60例宫颈癌活检组织人乳头瘤病毒的检测,结果表明,多重引物PCR检出率为81.66％;共有引物PCRHPV检出率为85％,两法符合毕为93.33％。共有引物PCR法比多重引物PCR法检出率高。两种方法各有其特点。本文认为大规模普查最好采用共有引物PCR法,它的特点是广谱,费用低。对临床HPVs感染的鉴别诊断最好选用多重PCR法。
Human cervical squamous cell carcinoma (referred to as cervical cancer) is one of the major causes of death among women. In recent years, laboratory studies have demonstrated that certain types of human papillomavirus (HPV) and cervical cancer and other cancers Reproductive oncology disease has a clear relationship. In this paper, the use of multiple primers and primers PCR method of 60 cases of cervical cancer in Shanxi Province of human papillomavirus detection, the results showed that the multiplex PCR detection rate was 81.66%; PCRHPV common primer PCR was 85%, the two methods are in line Completion of 93.33%. The common primer PCR method has higher detection rate than multiplex primer PCR method. Both methods have their own characteristics. In this paper, it is suggested that the common primer PCR method is most suitable for large scale census, which is characterized by broad spectrum and low cost. The differential diagnosis of clinical HPVs infection is best to use multiple PCR method.