论文部分内容阅读
目的研究珍珠菜全草乙酸乙酯提取物中的五环三萜-3-O-对香豆酸酯类化学成分。方法利用硅胶柱色谱法、Sepha-dex LH-20凝胶柱色谱法、中压柱色谱及半制备高效液相色谱等方法分离纯化;通过核磁共振谱、质谱、红外光谱等光谱数据鉴定化合物结构。结果分离得到6种五环三萜-3-O-对香豆酸酯类化合物,分别被鉴定为(Z)-马斯里酸-3-O-对香豆酸酯(Ⅰ),(E)-马斯里酸-3-O-对香豆酸酯(Ⅱ),(Z)-2α-羟基乌苏酸-3-O-对香豆酸酯(Ⅲ),(E)-2α-羟基乌苏酸-3-O-对香豆酸酯(Ⅳ),(Z)-萎陵菜酸-3-O-对香豆酸酯(Ⅴ),(E)-萎陵菜酸-3-O-对香豆酸酯(Ⅵ)。结论化合物Ⅰ~Ⅵ均为首次在珍珠菜属植物中发现。
Aim To study the chemical constituents of pentacyclic triterpene-3-O-p-coumarate in ethyl acetate extract of whole plant of Lilium petiole. Methods The compounds were isolated by silica gel column chromatography, Sephadex LH-20 gel column chromatography, medium pressure column chromatography and semi-preparative high performance liquid chromatography. The structures of the compounds were identified by 1H NMR, MS and IR spectra . Results Six pentacyclic triterpene-3-O-p-coumaric acid esters were isolated and identified as (Z) -malezole-3-O-p-coumarate (Ⅰ) 3-O-p-coumarate (II), (Z) -2α-hydroxyoxysulphone-3-O-p-coumarate (III), (E) -2α- 3-O-p-coumarate (Ⅳ), (Z) -Zincinate-3-O-p-coumarate (Ⅴ), -O-p-coumarate (Ⅵ). Conclusion Compounds Ⅰ ~ Ⅵ were found for the first time in the genus.