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目的 探讨地塞米松对实验性变态反应性脑脊髓炎 (EAE)小鼠胸腺细胞凋亡的影响。方法 采用昆明小鼠建立 EAE模型 ,于处死前 2 4小时腹腔注射地塞米松 ,通过荧光染色法、原位末端标记法和电镜法观察地塞米松处理组、自然病程组和对照组的小鼠胸腺细胞凋亡。结果 地塞米松处理组的胸腺细胞凋亡率明显高于自然病程组和正常对照组 (P<0 .0 1) ;且电镜发现有典型的凋亡改变。结论 地塞米松可明显增强 EAE小鼠胸腺细胞凋亡。
Objective To investigate the effect of dexamethasone on thymocyte apoptosis in experimental allergic encephalomyelitis (EAE) mice. Methods The model of EAE was established in Kunming mice. Dexamethasone was injected intraperitoneally 24 hours before the death. The mice in dexamethasone group, control group and natural history group were observed by fluorescence staining, in situ end labeling and electron microscopy. Thymocyte apoptosis. Results The apoptosis rate of thymus cells in dexamethasone treated group was significantly higher than that in natural course group and normal control group (P <0.01). The typical apoptotic changes were found by electron microscopy. Conclusion Dexamethasone can significantly enhance the apoptosis of thymocytes in EAE mice.