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目的： 构建含猪囊尾蚴抗原ｃ Ｃ１ ｃ Ｄ Ｎ Ａ 与猪γ干扰素（ Ｉ Ｆ Ｎγ） 的融合表达载体。方法： 从已克隆的含人猪囊尾蚴共通抗原ｃ Ｃ１ 的质粒中， 用 Ｐ Ｃ Ｒ 方法扩增出含酶切位点及接头的ｃ Ｃ１ 和 Ｉ Ｆ Ｎγｃ Ｄ Ｎ Ａ， 两ｃ Ｄ Ｎ Ａ 经接头融合后构建成融合形式的原核表达载体转入大肠杆菌 Ｘ Ｌ Ｂｌｕｅ。结果： 经酶切分析， 表明重组载体中含１５ｋｂ 插入片段。 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 显示一５２ ｋ Ｄａ 的诱导产物。结论： 猪 Ｉ Ｆ Ｎγ和猪囊尾蚴抗原ｃ Ｃ１ 融合ｃ Ｄ Ｎ Ａ 在大肠杆菌中获得表达。
OBJECTIVE: To construct a fusion expression vector containing cysticercus cellulosae antigen c C1 c D N A and porcine interferon γ (IFNγ). Methods: From the cloned plasmids containing the common antigen c C1 of human Cysticercus cellulosae, c C1 and I F Nγc D N A with restriction enzyme sites and linkers were amplified by PCR method, N A was fused to construct a fusion prokaryotic expression vector into E. coli X LBlue. Results: Restriction analysis showed that the recombinant vector contained 1 5kb insert. S D S-P A G E showed a 52 kDa induction product. Conclusions: Pig I F N γ and cysticercus cellulosae c C1 fusion c D N A were expressed in E. coli.