在日本血吸虫病非流行区，选无疫水接触史，８个月龄左右的水牛，用日本血吸虫重组ＧＳＴ抗原进行免疫观察对水牛的保护性。结果显示，攻击感染血吸虫尾蚴后，实验组比对照组平均虫荷减少２２．３％，粪卵ＥＰＧ差异有显著意义，毛蚴孵出率降低近４０％，肝、肠组织血吸虫卵沉积量减少４７．９４％～５６．８３％。 In the non-endemic area of ​​schistosomiasis japonica, buffalos were immunized with recombinant GST antigen of Schistosoma japonicum for buffaloes, which had no history of exposure to water and buffaloes of 8 months old. The results showed that the average worm burden decreased by 22.3% in the experimental group compared with the control group after the attack of Schistosoma japonicum cercariae. The difference of EPG in the fecal eggs was significant, the hatching rate of miracidia was reduced by 40%, and the deposition of schistosome eggs in the liver and intestinal tissues decreased by 47% .94% ~ 56.83%.