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由丝核菌引起的根腐病是草莓生产上的重要病害之一。本研究基于形态学、菌丝细胞核荧光染色、菌丝融合群测定、rDNA-ITS序列分析和柯赫氏法则验证,对北京地区引起草莓根腐病的丝核菌进行了鉴定。2014年从北京市昌平区温室草莓根腐病病样中分离纯化获得的3个代表菌株,经形态学和细胞核荧光染色,确定均为双核丝核菌(binucleate Rhizoctonia,BNR),且与双核丝核菌AG-A融合群菌株发生菌丝融合,菌株CP-Z的rDNA-ITS序列与GenBank中丝核菌属的有性型角担菌属(Ceratobasidium)AG-A融合群4个菌株的相似性达100%。菌株CP-Z接种草莓根部,引起根系变黑、腐烂,植株死亡,从接种发病的根部可重新分离到双核丝核菌。双核丝核菌AG-A融合群引起草莓根腐病为国内首次报道。该病原菌菌丝生长适温为25℃~28℃。
Root rot caused by Rhizoctonia solani is one of the most important diseases in strawberry production. In this study, Rhizoctonia rufipogon that caused strawberry root rot in Beijing was identified based on morphology, mycelium nuclear staining, mycelial fusion population, rDNA-ITS sequence analysis and Koch’s rule validation. In 2014, three representative strains isolated and isolated from strawberry root rot samples in greenhouse in Changping District of Beijing were identified as binucleate Rhizoctonia (BNR) by morphological and nuclear staining, Mycobacterium AG-A fusion group mycelial fusion occurs, the strain CP-Z rDNA-ITS sequence and GenBank Rhizoctonia genus Actinomyces (Ceratobasidium) AG-A fusion group of four strains of similar 100% sexual. Strain CP-Z inoculated strawberry roots, causing the root blackening, decay, plant death, from the inoculation of the roots can be re-isolated to Rhizoctonia. The first root cause of strawberry root rot in R. solani AG-A fusion group was reported in China. The pathogen mycelium growth suitable temperature is 25 ℃ ~ 28 ℃.