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目的:建立HPLC测定血络通胶囊中人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1含量的方法.方法:采用HPLC法,色谱柱为Waters Symmetry C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱,流速为1.0 ml·min-1,检测波长为203 nm,柱温为35℃,进样量为10μl.结果:人参皂苷Rg1在0.055~2.732μg(r=0.9998)范围内线性关系良好,平均加样回收率为107.23%,RSD为1.17%(n=6);人参皂苷Re在0.342~8.542μg(r=0.9999)范围内线性关系良好,平均加样回收率为101.63%,RSD为3.52%(n=6);人参皂苷Rb1在0.717~14.336μg(r=0.9997)范围内线性关系良好,平均加样回收率为100.63%,RSD为3.79%(n=6).结论:该方法简便、准确、可靠,可用于血络通胶囊的质量控制.“,”Objective:To establish an HPLC method for the determination of ginsenoside Rg1 ,ginsenoside Re and ginsenoside Rb1 in Xueluotong capsules. Methods:A column of Waters Symmetry C18 ( 250 mm × 4. 6 mm,5 μm) at the temperature of 35 ℃ was used to separate the target components, the mobile phase consisted of acetonitrile-water with gradient elution, the flow rate was 1. 0 ml ·min-1 , the detection wavelength was 203 nm and the injection volume was 10 μl. Results:The linear range of ginsenoside Rg1 was 0. 055-2. 732 μg(r=0. 9998), and the average recovery was 107. 23% with RSD of 1. 17%(n=6). The linear range of ginsenoside Re was 0. 341-8. 542 μg(r=0. 9999), and the average recovery was 101. 63% with RSD of 3. 52%(n=6). The linear range of gin-senoside Rb1 was 0. 717-14. 336 μg(r=0. 9997), and the average recovery was 100. 63% with the RSD of 3. 79%(n=6). Conclu-sion:The method is simple, accurate and reliable, which can be used for the quality control of Xueluotong capsules.