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为了测定田七花总皂苷中人参皂苷Rb1的含量,试验采用HPLC-MS/MS分析方法,以BDS HYPERSUL C18(150 mm×2.1 mm,5μm)柱为色谱柱;乙腈-水(梯度洗脱)为流动相;柱温为30℃;检测模式为多反应离子监测模式(MRM),用于定量分析的对照品离子对为人参皂苷Rb1(m/z1 107.9→783.7),内标物紫杉醇(m/z 852.5→525.3)。结果表明:人参皂苷Rb1标准曲线的线性范围为0.159~16.0μg/m L,精密度和准确度等均符合样品分析要求。说明该方法准确、灵敏、特异性强,适用于田七花总皂苷及其制剂中人参皂苷Rb1浓度的检测。
In order to determine the content of ginsenoside Rb1, the HPLC-MS / MS method was used to determine the content of ginsenoside Rb1. The chromatographic column was BDS HYPERSUL C18 (150 mm × 2.1 mm, 5 μm) As the mobile phase. The column temperature was 30 ℃. The detection mode was MRM. The standard ion pair for quantitative analysis was ginsenoside Rb1 (m / z1 107.9 → 783.7) / z 852.5 → 525.3). The results showed that the linear range of the standard curve of ginsenoside Rb1 was 0.159 ~ 16.0 μg / mL, and the precision and accuracy were in accordance with the requirements of sample analysis. This method is accurate, sensitive and specific. It is suitable for the determination of ginsenoside Rb1 in total saponins of Panax notoginseng and its preparation.