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卵泡的颗粒细胞和卵泡膜细胞分离较易,故对其性激素合成研究较多。黄体大部分由粒层黄体细胞(GL)所构成,但周围有极少数卵泡膜黄体细胞(TL),两者之间不存在作为分界的基底膜,故用机械方法将两者完全分离是极为困难的。这成为对该领域进行研究的一大障碍。为了克服方法学上的制约,日本的新居延健二用12例人类成熟期黄体和初期妊娠黄体作实验,将黄体分成二种薄片,内侧薄片仅含GL,外侧薄片则GL和TL都含。在加入各种底物后,再比较两者的性甾
Follicular granulosa cells and follicular cells isolated more easily, so the synthesis of sex hormones more. Most of the corpus luteum is composed of granular luteal cells (GL), but there are very few surrounding luteal cells (TL) around the corpus luteum, and there is no basement membrane between them as the boundary. Therefore, the complete separation of the two bodies by mechanical means is extremely difficult. This has been a major stumbling block to research in this area. In order to overcome the methodological constraints, Japan’s new home Yanjian II with 12 cases of human mature luteal and primary pregnancy luteal for the experiment, the luteal is divided into two kinds of slices, the inner slice only GL, outer slice GL and TL are contained. After adding a variety of substrates, and then compare the two sex steroids