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目的研究1型糖尿病(T1DM)小鼠胰岛β细胞量的变化及变化机制。方法糖尿病第4、12、20周时,T1DM小鼠被放血处死。以Insulin、Kruppel-like factor 4(KLF4)、Caspase-3、TUNEL的免疫荧光标记及再生蛋白1 mRNA半定量RT-PCR检测β细胞量、β细胞再生和凋亡。结果β细胞量随糖尿病发病时间延长逐渐减少,至糖尿病第12周时降至最低,第20周与第12周比较无统计学差异。β细胞量的变化与其Caspase-3、KLF4的表达和胰腺再生蛋白1 mRNA的丰度密切相关。结论 1型糖尿病鼠胰岛β细胞并未完全丧失,其机制与β细胞凋亡减少、再生增加密切相关。
Objective To study the changes and mechanisms of pancreatic β-cell mass in type 1 diabetic mice (T1DM). Methods At the 4th, 12th and 20th week of diabetes mellitus, T1DM mice were sacrificed by exsanguination. Immunofluorescent labeling of Insulin, Kruppel-like factor 4 (KLF4), Caspase-3 and TUNEL, and semiquantitative RT-PCR of regenerative protein 1 mRNA were used to detect β-cell mass, β cell regeneration and apoptosis. Results The amount of β-cells decreased gradually with the onset of diabetes and reached the lowest level at the 12th week of diabetes. There was no significant difference between the 20th week and the 12th week. The change of β-cell mass is closely related to the expression of Caspase-3, KLF4 and the abundance of Pancreatic-1 protein. Conclusion The islet β cells of type 1 diabetic mice are not completely lost, and the mechanism is related to the decrease of β-cell apoptosis and the increase of regeneration.